Prevalence Of Cryptosporidium Research Articles

Cryptosporidium disease of small ruminants in the region of El Taref and Mostaghanem

In order to identify and quantify Cryptosporidium infection in small ruminants in northern Algeria, specifically in the regions of Tarf and Mostaganem, a three-year survey was conducted between 2019 and 2021. The epidemiological investigation covered several aspects of the prevalence and etiology of Cryptosporidium, including various factors suspected to contribute to the spread of the causative agent of this disease. In addition, a confirmatory study was initiated, with bacteriological and histopathological tests performed on samples collected during the survey period from the various farms examined. The results showed that the epidemic takes several aspects for its spread as well as its persistence, the bacteriological results showed a coincidence of the presence of the pathogen in a significant proportion, and the histopathological interpretation of the slides revealed the diversity of lesions at the intestinal level with the predominance and inversion of enterocytes by sporozoites, resulting in the recurrence of lesions in the different organs collected in all species studied, cattle, sheep and goats.

Prevalence and molecular characterization of Cryptosporidium spp., Enterocytozoon bieneusi, and Giardia duodenalis in dairy cattle in Ningxia, northwestern China.

Cryptosporidium spp., Enterocytozoon bieneusi, and Giardia duodenalis are common intestinal pathogens that infect humans and animals. To date, research regarding these three protozoa in the Ningxia Hui Autonomous Region (Ningxia) has mostly been limited to a single pathogen, and comprehensive data on mixed infections are unavailable. This study aimed to evaluate the zoonotic potential of these three protozoa. In this study, small subunit ribosomal RNA (SSU rRNA) and 60 kDa glycoprotein (gp60) genes of Cryptosporidium; internal transcribed spacer (ITS) gene of E. bieneusi; and SSU rRNA, glutamate dehydrogenase (gdh), triosephosphate isomerase (tpi), and beta-giardin (bg) genes of G. duodenalis were examined. DNA extraction, polymerase chain reaction, and sequence analysis were performed on fecal samples collected from 320 dairy cattle at three intensive dairy farms in Ningxia in 2021 to determine the prevalence and genetic characteristics of these three protozoa. The findings revealed that 61.56% (197/320) of the samples were infected with at least one protozoan. The overall prevalence of Cryptosporidium was 19.38% (62/320), E. bieneusi was 41.56% (133/320), and G. duodenalis was 29.38% (94/320). This study identified four Cryptosporidium species (C. bovis, C. andersoni, C. ryanae, and C. parvum) and the presence of mixed infections with two or three Cryptosporidium species. C. bovis was the dominant species in this study, while the dominant C. parvum subtypes were IIdA15G1 and IIdA20G1. The genotypes of E. bieneusis were J, BEB4, and I alongside the novel genotypes NX1-NX8, all belonging to group 2, with genotype J being dominant. G. duodenalis assemblages were identified as assemblages E, A, and B, and a mixed infection involving assemblages A + E was identified, with assemblage E being the dominant one. Concurrently, 11 isolates formed 10 different assemblage E multilocus genotypes (MLGs) and 1 assemblage A MLG and assemblage E MLGs formed 5 subgroups. To the best of our knowledge, this is the first report on mixed infection with two or three Cryptosporidium species in cattle in Ningxia and on the presence of the C. parvum subtype IIdA20G1 in this part of China. This study also discovered nine genotypes of E. bieneusis and novel features of G. duodenalis assemblages in Ningxia. This study indicates that dairy cattle in this region may play a significant role in the zoonotic transmission of Cryptosporidium spp., E. bieneusi, and G. duodenalis.

Review on Bovine Cryptosporidiosis, its Associated Risk Factors and Diagnostics Methods

Bovine cryptosporidiosis, caused by the protozoan parasite Cryptosporidium, poses a significant threat to cattle health and productivity worldwide. This review provides a comprehensive analysis of bovine cryptosporidiosis, focusing on its associated risk factors and diagnostic methods. Understanding the risk factors contributing to the prevalence and transmission of Cryptosporidium in cattle populations is crucial for effective disease management. Factors such as age, herd size, housing conditions, environmental contamination, and geographic location play pivotal roles in the epidemiology of bovine cryptosporidiosis. Additionally, interactions between host susceptibility and pathogen virulence further complicate disease dynamics.

Cryptosporidium and associated risk factors in local breeds of pigs kept in Donga Local Government Area Taraba State, Nigeria

Cryptosporidium, an enteric protozoan parasite, poses economic losses in animal production and has zoonotic potential. This study aimed to determine the prevalence of cryptosporidium and associated risk factors in local pig breeds within Donga Local Government Area of Taraba State, Nigeria. A cross-sectional study was conducted, and a total of 384 faecal samples were collected from pigs in eight sampling sites. The samples were examined using the modified Ziehl-Neelsen staining technique.The results showed an overall prevalence of 27.9% (107/384) for cryptosporidium oocysts. The prevalence was significantly higher in pre-weaned piglets (64%) compared to post-weaned (26.9%) and adult pigs (24.14%). Female pigs had a significantly higher prevalence (49.21%) than males (17.4%), and diarrhoeic pigs had a significantly higher prevalence (55.2%) than non-diarrhoeic (22.9%). The prevalence was higher in the rainy season (42.4%) than in the dry season (10.3%).The detection of Cryptosporidium spp. oocysts in the faeces of pigs suggests that these animals may serve as potential reservoirs and shedders of this protozoan parasite, contaminating the environment and serving as a source of infection to other animals and humans. Adequate personal and environmental hygiene, along with prompt veterinary care, should be intensified.

Cryptosporidium spp. and Eimeria spp. (Apicomplexa: Eimeriorina) of freshwater Cyprinid fish species in the Kura River basin in Azerbaijan territory.

This study aims to determine the prevalence of Cryptosporidium and Eimeria spp. oocysts in fish specimens in the river Kura. It was conducted during the 2021-2022 at two sites: Mingachevir reservoir in central Azerbaijan and in Neftchala district where the river finally enters the Caspian Sea through a delta of the Kura River estuary. The diagnosis of oocysts was performed microscopically. Fine smears from the intestine epithelial layers stained by Ziehl-Neelsen for Cryptosporidium oocysts. To identify Eimeria oocysts, each fish's faecal material and intestinal scrapings were examined directly under a light microscope in wet samples on glass slides with a coverslip. Results revealed a prevalence of Cryptosporidium and Eimeria species infections in fish hosts from both territories Rutilus caspicus, Alburnus filippi, Abramis brama orientalis and Carassius gibelio. Of 170 investigated fish specimens, 8.8% (15/170) were infected with Cryptosporidium species oocysts. Eimeria species oocysts were identified in 20.6% (35/170). The presence of Cryptosporidium and Eimeria infections in fish specimens are natural infections. However, their presence in fish species may be attributed to the age of the fish species and water pollution. This is the first report regarding the prevalence of Cryptosporidium oocysts in fish species in Azerbaijan.

Molecular characterization of Cryptosporidium spp. and Giardia duodenalis in pet cats in Henan Province, central China

Cryptosporidium spp. and G. duodenalis often infect humans, cats, and other mammals, causing diarrhea and being responsible for numerous outbreaks of waterborne and foodborne infections worldwide. The rapid increase in the number of pet cats poses a substantial public health risk. However, there were few reports about the infection of Cryptosporidium spp. and G. duodenalis infections in pet cats in Henan Province, central China. Thus, to understand the prevalence and genetic distribution of Cryptosporidium spp. and G. duodenalis in pet cats, and to evaluate the zoonotic potential, possible transmission routes and public health implications of isolates, fecal samples (n = 898) were randomly collected from pet cats in 11 cities in Henan Province, central China. Nested PCR based on the SSU rRNA gene and bg gene was used to the prevalence of Cryptosporidium spp. and G. duodenalis, respectively. The prevalence was 0.8 % (7/898) and 2.0 % (18/898) for Cryptosporidium spp. and G. duodenalis respectively. Additionally, the Cryptosporidium spp. positive isolates were identified as C. parvum subtype IIdA19G1 by gp60 gene. In the present study, the IIdA19G1 subtype was discovered in pet cats for the first time in China, enriching the information on the host type and geographical distribution of Cryptosporidium spp. in China. For G. duodenalis, a total of 18 G. duodenalis positive samples were identified, belonging to four assemblages: a zoonotic assemblage A1 (4/898), three host-specific assemblages C (8/898), D (5/898), and F (1/898). Interestingly, we found that pet cats infected with Cryptosporidium spp. and G. duodenalis are more likely to experience emaciation symptoms compared to the negative group. More importantly, the prevalence of Cryptosporidium spp. and G. duodenalis detected in the present study were low, but the subtype IIdA19G1 of Cryptosporidium spp. and the assemblages A1, C, D, and F of G. duodenalis have the potential for zoonotic transmission. Thus, we should focus on preventing and controlling the risk of cross-species transmission that may occur in pet cats in Henan Province.

Prevalence of Cryptosporidium spp. in Sheep and Goats in Jiangsu, China.

Sheep and goats serve as crucial hosts for Cryptosporidium spp. and are primarily responsible for its transmission via the fecal-oral route. This can result in symptoms such as lamb weight loss, diarrhea, and even fatalities, leading to significant economic losses. Currently, there is a lack of scholarly research investigating the prevalence of Cryptosporidium spp. infection in sheep and goats specifically within Jiangsu province. This study collected fecal samples from sheep and goats, extracted their DNA, amplified target bands using nested PCR, sequenced the DNA, constructed a phylogenetic tree, and identified the genetic genotype. In total, 3 positive samples were identified out of 398 samples. Furthermore, the gene sequences of these samples exhibited significant homology with C. xiaoi in GenBank. The phylogenetic analysis revealed that the Cryptosporidium spp. parasites under investigation are phylogenetically related to C. xiaoi. Conducting epidemiological investigations and accurately identifying the species of Cryptosporidium spp. is of utmost importance not only for the mutton sheep farming industry in Jiangsu but also for the proactive safeguarding of human health.

Epidemiological investigation of Cryptosporidium in children with diarrhea in middle Inner Mongolia, China

Cryptosporidia (Cryptosporidium) is a protozoan that is widely parasitic in the intestinal cells of humans and animals, and it is also an important zoonotic parasite. However, there is no epidemiological investigation on Cryptosporidium spp. infection in infants with diarrhea of Inner Mongolia, the largest livestock region in China. To investigate the prevalence of Cryptosporidium, 2435 fresh fecal samples were collected from children with diarrhea in Inner Mongolia Maternal and Child Health Care Hospital. Molecular characterization of Cryptosporidium was carried out based on its 18S rRNA and gp60 gene sequences. The overall prevalence was 12.85% (313/2435), and in Hohhot (12.15%), it was lower than that in the surrounding city (14.87%) (P < 0.05). Moreover, Cryptosporidium was detected in different seasons and sexes. Concerning the age of children with diarrhea, the prevalence of those age groups between 0 and 1 was obviously lower than others, and there were significant differences in the prevalence at different ages (P < 0.001). Analysis of the 18S rRNA gene sequence revealed that all the positive samples were Cryptosporidium parvum, and there were 5 subtypes (IIdA23G3, IIdA24G3, IIdA24G4, IIdA25G3, and IIdA25G4). To the best of our knowledge, the above subtypes have not been reported. Our results provide a relevant basis for control and education on food safety and foodborne illness prevention.

Molecular Characterization of Giardia intestinalis and Cryptosporidium spp. Detected in Humans in Ağrı, Türkiye.

We aimed to determine the prevalence of Giardia intestinalis and Cryptosporidium spp. in patients who admitted hospital with diarrhea and to gain information about the transmission of these parasites in Ağrı, Türkiye. This study included 184 patients who applied to Ağrı-Diyadin State Hospital, Türkiye in 2022. The immunochromatographic card test was used for detection of the G. intestinalis and Cryptosporidium spp. Nested PCR-RFLP analysis of the COWP gene and sequence analysis of the gp60 gene were used to genotype and subtype Cryptosporidium spp., whereas Nested PCR and sequence analyses of β-giardin gene were used genotype G. intestinalis. Of the 184 stool specimens examined, 12 (14.29%) and 7 (3.80%) were positive for G. intestinalis and Cryptosporidium spp., respectively. The Cryptosporidium species were identified as C. parvum belonging to the IId sub-type family. The G. intestinalis were identified assemblages A. Assemblage A, which is associated with diarrhea, is responsible for giardiasis and C. parvum IId subtype, often found in sheep, goats and cattle, is responsible for cryptosporidiosis in Ağrı, Türkiye.

Prevalence and potential risk factors for Cryptosporidium spp. infection in horses from Egypt

Cryptosporidium is an intestinal protozoan that cause diarrhea in livestock all over the world and have zoonotic importance. The present study aimed to determine the prevalence of Cryptosporidium spp. in horses in Egypt and evaluate the associated risk factors. A total of 420 fecal samples were collected from three governorates (Giza, Kafr ElSheikh and Qalyubia) and examined microscopically using Ziehl-Neelsen staining method. The overall prevalence of Cryptosporidium spp. was 29% and Kafr Sheikh governorate had the highest rate in comparison to other areas. The prevalence of Cryptosporidium spp. in examined horses had significant association with sex, age, type of management, absence of bedding and presence of dogs. The higher prevalence rate was observed in females (32.2%), age group less than two years (43.2%), mixed (grazing and stable) horses (36.1%), animals had history of diarrhea (33%), absence of bedding (35.1%) and contact with dogs (35.7%). These findings give baseline data for further research. It is necessary to establish control strategy for Cryptosporidium spp. infection in order to lower the risk of infection in animals and human.

Effect of Dietary Supplementation of Chestnut and Quebracho Tannin Supplementation on Neonatal Diarrhoea in Preweaning Calves.

Neonatal calf diarrhoea (NCD) poses a significant health challenge in cattle herds, resulting in considerable economic losses and antimicrobial use. In response to the escalating threat of antimicrobial resistance, viable alternatives are imperative, aligning with European policies. This study evaluated the in-milk supplementation of the chestnut and quebracho tannin extract in preweaning calves on performance, diarrhoea occurrence, Cryptosporidium spp. shedding, protein digestibility, and intestinal health. Twenty newborn calves were divided, after colostrum administration, into two experimental groups for 30 days as follows: the control (CTRL) was fed with whole milk and solid feed, and tannins (TAN) were fed whole milk supplemented with 6/g day of tannin extract and solid feed. Faecal samples were collected on days 0, 3, 7, 14, and 30 for the evaluation of Cryptosporidium oocyst shedding and protein digestibility. Faecal consistency was evaluated during the sampling using the faecal score scale (0-3 scale, considering diarrhoea > 1). The results showed a significant reduction in diarrhoea frequency in the TAN compared to the CTRL group (p < 0.05) over 30 days of the trial. The prevalence of Cryptosporidium spp. was generally low (12%), considering all analysed samples. Protein digestibility revealed comparable values for the TAN and CTRL groups, suggesting that tannins did not negatively affect milk protein availability. In conclusion, the in-milk supplementation of 6/g day of the chestnut and quebracho tannin extract could be considered a valuable functional feed additive to decrease NCD occurrence, thus supporting animal health and decreasing antibiotic use in livestock.

ASSESSMENT OF WATERBORNE PARASITES IN DRINKING WATER SOURCES IN IRAQ

Background and Aim:Waterborne parasites present substantial health hazards, hence requiring extensive research efforts to ascertain their prevalence and spatial dispersion. The objective of this study is to examine the prevalence of Cryptosporidium spp., Entamoeba histolytica, and Giardia lamblia in samples of river and municipal water collected from various geographical areas. The main objective of this study is to evaluate and contrast the occurrence of waterborne parasites in various drinking water sources, thereby offering valuable insights into spatial disparities and facilitating the development of focused strategies for managing water quality. Materials and Methods:Water samples were obtained from four distinct geographical areas, namely Al-Awja, Amarah, Tikrit, and Sultan Abdullah, which encompass both river and urban water sources. The detection of parasites was carried out utilizing established protocols. Results: Among the many water samples collected from rivers, it was observed that Al-Awja demonstrated the greatest overall prevalence rate of 53.33%. Notably, the municipal water in Al-Awja and Amarah exhibited considerable rates of 17.77% and 4.44% respectively. The major species observed were Cryptosporidium spp. and Giardia lamblia. There was a positive correlation observed between parasite prevalence and seasons in both river water samples (0.019567) and city water samples (0.023613). The correlation coefficient of 0.987524 indicates that the observed relationship may lack statistical significance between Parasites prevalence and sources of water. Conclusion: Waterborne parasites are common, highlighting the need for tailored water quality control. The increased frequency in certain locations emphasises the need for public health surveillance and informed responses. This research advances our understanding of waterborne parasite spatial dynamics, guiding water safety and community health measures.

Prevalence of Cryptosporidium and Giardia infections in under-five children with diarrhoea in Blantyre, Malawi

BackgroundDiarrhoeal diseases are common among children in low- and middle-income countries and are major causes of morbidity and mortality. Cryptosporidium and Giardia are considered to be the main parasitic causes of diarrhoea in children. The aim of the present study was to determine the prevalence and associated factors of Cryptosporidium and Giardia infection in children under five years of age presenting at two health centres (Ndirande and Limbe) in Blantyre, Malawi.MethodsThis cross-sectional study was performed from February to July 2019 and included 972 children under 5 years of age with diarrhoea. Stool samples were immediately tested after collection at enrolment with a rapid diagnostic test for Cryptosporidium and Giardia infection. Descriptive statistics were used to assess the prevalence of these protozoan parasitic infections, and differences in the basic demographic and anthroponotic variables (between children with diarrhoea and parasite infection, being either Cryptosporidium and Giardia or both versus children with diarrhoea but no RDT confirmed parasite infection) were assessed. Their association with Cryptosporidium and Giardia infection was analysed using simple logistic regressions.ResultsOf the children recruited, 88 (9.1%) tested positive for Cryptosporidium and 184 (18.9%) for Giardia. Children with only a Giardia infection or a coinfection (of both parasites) were significantly older (mean age 24–26 months) compared to children with only a Cryptosporidium infection (mean age 13 months) or no parasitic infection (mean age 14 months). No significant differences were found with respect to gender, body temperature, stunting or wasting between the different groups of children with moderate to severe diarrhoea. Children attending the Ndirande health centre had almost two times higher odds of testing positive for both infections than those attending Limbe health centre.ConclusionCryptosporidium and Giardia infections are highly prevalent in children < 5 years with moderate to severe diarrhoea attending the Limbe and Ndirande health centres in Blantyre, Malawi.

Cryptosporidium species diagnosis in handlers of domestic pigeons in Baghdad City: Molecular and microscopic approaches.

The aim of this study is to detect the prevalence and diagnosis of molecular characteristics of Cryptosporidium infection in handlers of domestic pigeons in Baghdad City. Traditional and molecular diagnostic methods were employed to detect and identify Cryptosporidium species. Sixty stool samples were collected from the handlers of domestic pigeons, and various techniques, including direct smear, flotation concentration, staining methods, and DNA extraction coupled with nested polymerase chain reaction (PCR), were utilized. The results obtained from traditional methods indicated an overall infection rate of 55% in handlers of domestic pigeons, while significant variations were noted between male and female handlers. Age group 21-40 years were found to have higher infection rates were found to have higher infection rates. The prevalence of Cryptosporidium also displayed temporal variability throughout the study period. Molecular analysis using nested PCR revealed higher infection rates of 86% in handlers of domestic pigeon samples. Genotyping and phylogenetic analysis identified the presence of Cryptosporidium parvam species in handlers of domestic pigeons, indicating zoonotic transmission potential. These findings underscore the high prevalence of Cryptosporidium infection among handlers of domestic pigeons in Baghdad City.

Investigation of Factors Associated with Subclinical Infections of Giardia duodenalis and Cryptosporidium canis in Kennel-Housed Dogs (Canis lupus familiaris).

Giardia duodenalis and Cryptosporidium spp. are zoonotic protozoal pathogens, spread by a fecal-oral route, which can infect a wide range of hosts including but not limited to dogs and humans. Giardia was recently estimated to be present in 37% to 50% of kennel-housed dogs. Cryptosporidium infections in kennel-housed dogs have been reported in 7% to 21% of the population. The goal of this study was to define demographic factors and fecal scores associated with positive screening test cases of Giardia and Cryptosporidium in kennel-housed laboratory dogs in the state of Texas. Fecal samples were collected from 153 clinically normal laboratory dogs at an academic research facility and a local laboratory dog supplier. We used 3 diagnostic tests evaluated in parallel to determine test positivity to each organism: a human point-of-care coproantigen test, a direct immunofluorescent assay, and an in-house polymerase chain reaction. Dogs were significantly more likely to test positive for Giardia (45%) than Cryptosporidium (7%) (P < 0.01). Dogs that were 18 mo of age or younger had 3 times the odds (P = 0.009) of subclinical Giardia infection compared with older dogs. We found no significant relationship between age and Cryptosporidium prevalence. Dogs with hard feces (fecal score 1-2) at the time of screening had 0.34 times lower odds (P = 0.049) of testing positive for Giardia than dogs with normal feces, but no statistically significant relationship was found between fecal score and Cryptosporidium-positive test status. With these findings, we demonstrated the value of considering age and fecal score when choosing which dogs to screen for subclinical Giardia. Additional studies with larger sample sizes should be conducted to determine the relationship between age and fecal score and subclinical Cryptosporidium infection.

The prevalence of Cryptosporidium spp. and other intestinal parasites in elderly patients

Purpose: Cryptosporidiosis is a self-limiting infection in individuals with immune competence, but it can have serious and life-threatening consequences in the elderly and immune-compromised individuals. In this study, we aimed to investigate the prevalence of Cryptosporidium spp. and other intestinal parasites and their relationship with symptoms in elderly patients.&#x0D; Materials and Methods: The distribution of Cryptosporidium spp. and other intestinal parasites was prospectively evaluated in elderly patients admitted to different clinics of the university hospital between September 2018 and September 2019. &#x0D; The study group included 40 elderly patients (18 females and 22 males; mean age: 73.35 ±6.55years) with gastrointestinal complaints and a control group consisting of 44 elderly patients without gastrointestinal complaints. Stool samples were examined using wet mount, and Kinyoun acid fast staining.&#x0D; Results: The most frequently isolated parasites were amoeba cysts and trophozoites in elderly patients. Cryptosporidium spp. cysts were detected in 9 (22.5%) of patients with gastrointestinal symptoms, and in 13 (40.6%) of those with other complaints and in the control group. Cryptosporidium spp. cysts were found more common in those with a solid stool consistency than others with a watery stool consistency.&#x0D; Conclusion: Cryptosporidiosis could be a life-threatening condition in a high-risk population, such as the elderly with co-morbidities, and physicians should have increased awareness.

Prevalence of Cryptosporidium and microsporidial infection in HIV-infected individuals.

Microsporidia and Cryptosporidium are obligate intracellular protozoa. These medically important species are recognized as opportunistic organisms in intestinal complications in human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome patients. The current cross-sectional study was designed and conducted from August 2016 to August 2017 to determine intestinal Cryptosporidium and microsporidia spp. in HIV-infected individuals from the Behavioral Diseases Counseling Center, Tabriz, Iran, by modified acid-fast and modified trichrome staining and nested polymerase chain reaction (PCR) and real-time PCR. Of 100 HIV-infected persons, 21.0% (95% confidence interval [CI] 13.0 to 30.0) and 18.0% (95% CI 11.0 to 26.0) were identified as Cryptosporidium and microsporidia, respectively, by the microscopic method. Of these 100 HIV-infected persons, 18.0% (95% CI 11.0 to 26.0) and 14.0% (95% CI 7.0 to 22.0) were positive for Cryptosporidium and microsporidia, respectively, by the molecular method. The predominant species of microsporidia in patients was Enterocytozoon bieneusi (85.7% [95% CI 57.0 to 98.0]) and Encephalitozoon cuniculi (14.3% [95% CI 1.7 to 42.0]), which were found by quantitative real-time PCR and its high-resolution melting tool. As far as we know, this study is the first to estimate the prevalence of infection with Cryptosporidium and microsporidia among HIV-infected persons in northwest of Iran. The prevalence of intestinal microsporidiosis and cryptosporidiosis in this area in HIV-infected people was higher than the global prevalence of infection among immunocompromised patients. In addition to the need for further studies to prove protozoan pathogenicity in the aforementioned group, preventive measures should be considered.

First Detection of Cryptosporidium parvum in the Endemic Cyprus Mouflon (Ovisgmelini ophion).

Cryptosporidiumis an intestinal zoonotic protozoan parasite that infects domesticated and wild animals. There are no reports on the prevalence and molecular characterisation ofCryptosporidiumin the endemic Cyprus mouflon. The mouflon is strictly protected by national and international legislation. Its main distribution is Paphos State Forest and surrounding areas, where it may share the same water sources as free-ranging domestic goats. Therefore, the present study aimed to determine the prevalence ofCryptosporidiumspp. and genotypes in mouflon and free-ranging goats within the mouflon range. Faecal samples of 70 mouflons and 34 free-ranging goats were screened forCryptosporidiumby PCR amplification and sequencing. Only one sample (1/70) belonging to a mouflon was PCR positive forCryptosporidium. Based on sequencing of the18SrRNA locus, this species was identified asCryptosporidium parvum (C. parvum). No positive sample was detected in the free-ranging goats (0/34). This is the first report on the molecular identification of thisCryptosporidiumspecies in a Cyprus mouflon. The results indicate that the prevalence of Cryptosporidium in Cyprus mouflon is low.

Prevalence and molecular characterization of Cryptosporidium spp. in dairy and beef cattle in Shanxi, China.

Cryptosporidium spp. are key gastrointestinal protists in humans and animals worldwide. Infected cattle are considered the main source of cryptosporidiosis outbreaks in humans. However, little is known about the genetic makeup of Cryptosporidium populations in Shanxi province, China. We analyzed 858 fecal samples collected from farms in Shanxi. The presence of Cryptosporidium spp. was determined via polymerase chain reaction and subsequent sequence analysis of the small subunit rRNA gene as well as restriction fragment length polymorphism analysis. Cryptosporidium parvum was subtyped following sequence analysis of the 60 kDa glycoprotein gene (gp60). The overall prevalence of Cryptosporidium in cattle was 11.19%, with a prevalence of 13.30% and 8.67% in Lingqiu and Yingxian, respectively. The overall prevalence of Cryptosporidium in dairy and beef cattle was 10.78% and 11.50%, respectively. Cryptosporidium infection was detected across all analyzed age groups. The overall prevalence of Cryptosporidium in diarrhea and nondiarrhea samples was 18.24% and 9.72%, respectively, whereas that in intensively farmed and free-range cattle was 17.40% and 3.41%, respectively. We identified five Cryptosporidium species, with C. andersoni being the dominant species. Further, two cases of mixed infections of Cryptosporidium species were detected. All identified C. parvum isolates belonged to the subtype IIdA17G1.

Molecular Characterization and Zoonotic Significance of Cryptosporidium spp. and Giardia duodenalis in Asymptomatic Adult Stray Cats and Dogs in Turkey

Background: Cryptosporidium species and Giardia duodenalis are important protozoan parasites due to their wide host range and potential as zoonotic diseases. These parasites, responsible for Cryptosporidiosis and Giardiasis, are significant health concerns and have garnered attention from both the public and veterinary fields. Pets, such as dogs and cats, are susceptible to these infections and play a crucial role in transmitting these protozoans to humans. This study was conducted to determine the prevalence, species, genotypes and zoonotic significance of Cryptosporidium and Giardia in asymptomatic adult stray cats and dogs. Methods: Fecal samples from 75 adult stray animal, 42 dogs and 33 cats, were collected in Diyarbakýr city, southeastern Anatolia, Turkey. Direct immunofluorescence test was used to confirm the presence of G. duoenalis cysts and cryptosporidium spp. oocysts in collected fecal samples. Microscopic analysis was used to count the number of cysts/oocysts per gram as specified by the manufacturer. Molecular confirmation was done with Polymerase chain reaction (PCR) using Giardia SSU-rDNA and Cryptosporidium spp. SSU rRNA gene. The secondary PCR products of all positive samples were sequenced in one directions on an automated sequencer. Nucleotide sequence analysis was performed by BLAST alignment using the National Center for Biotechnology Information database. Result: The prevalence of Cryptosporidium was 7.14% in 42 cats and 9.09% in 33 dogs. The prevalence of G. duodenalis was 23.80% in cats and 54.54% in dogs. The average number of Cryptosporidium spp. oocysts per gram of cat and dog feces was 1866.3 and 1813.3, respectively. The average number of G. duodenalis cysts per gram of cat and dog feces was 1011.6 and 4786, respectively. All Cryptosporidium isolates were determined as C. canis in dogs and C. felis in cats. C. canis and C. felis isolates identified in the study, MT329018.1 and MN696800.1, AF159113.1 and KM977642.1 showed similarity with Genbank number, respectively. Assemblages A, E, C, D were found in dogs and assemblages A and E in cats in the result of the sequence of 130 bp gene fragments obtained from G. duodenalis isolates. This study sequence analysis of G. duodenalis cat isolates identified assemblages A (50%/10) and E (50%/10). In this study, assemblage E was the most common genotype isolated in dogs (38.8%), followed by Assemblage C (27.7%), Assemblage D (16.6%) and Assemblage A (15.5%). Assemblage A, which has zoonotic importance, was detected in cats and dogs, while assemblage E in cats and dogs was reported for the first time in Turkey.